Thank you for breaking it down for those without a science background, talk about throwing the animals under the bus, is this agenda to rid the world of all living species beside humans? Nope not even going there
I find this post, and the one before on Bayesian analysis, to be the most strikingly objective pieces I've read during the pandemic. Everything else has a spin that can be felt. I sensed that you were truly open to letting the evidence lead you where it will. That's a *real* scientist... Thank you.
Thank you for explaining this to a laymen audience. Most of the arguments for a lab origin are perfectly understandable for everyone who is willing to do a little effort and keeping an open mind.
Presumably by primers with minimal overlap? One wonders what other systematic biases are introduced by such a kit. For example, they probably also down weight amplification of primates closely related to humans, and all manners of other animals may likely have variable alignments with their primer set, suggesting the actual relative abundances are meaningless as they are non-identifiably confounded by the artifact of primer binding intensity…
(The FastSelect kit), the same company that made the QIAmp viral RNA mini-kit “Qiagen micro-extraction kit”, enrichment of non-human reads are performed through the use of LNA oligomers that binds to and prevent amplification of nucleic acids carrying sequence homology as the oligomers in the kit.
The ILLUMINA Ribo-Zero Gold kit, which is the only available kit from illumina for the same purpose (human NA removal) before the release of the Ribo-Zero plus kit in 04/02/2020, use a probe based pulldown method to remove human rRNA, including mitochondrial rRNA. Both kits selectively removes human reads, and targets the mitochondrion.
Another important point is that the PCR assays developed from the work of Christian Drosten in Germany, and widely, if not universally used in the U.S. and elsewhere were not based upon actual genomic sequencing from a human or animal sample, but from a computer model! Cases, Cases! Cases up the wazoo!
They’re cute, though, those raccoon dogs. Most media outlets long ago lost their credibility, as well as social media. My question is, what exactly is this virus? Coronaviruses are ubiquitous in human and animal populations, and can make people sick, though usually not seriously so, and usually seasonally. There were only a few hot spots, with a relatively high fatality rate early on: Wuhan, Lombardy, NYC, the state of Washington. It appears that many of the deaths attributed to this virus throughout the clown show were iatrogenic, caused by the refusal of early treatment with proven re-purposed drugs like the zinc-ionophere hydroxycloroquine or the anti-parssitic ivermectin, the use of ventilators and remdisivir, and the change in the protocol for treating pneumonia (refusing to treat with antibiotics). Clearly, in these hot spots many of those hospitalized were very ill, but this was not the case in most of the world. Viral infectious diseases do not behave like this. Why would a virus behave very differently in a few places, and not everywhere else? Is it possible ,in a lab, to engineer a RNA virus to become both more lethal and easily transmissible? It seems to me a ridiculous idea, since, as you say, RNA is so unstable. Something clearly made those people sick in those hot spots, but what was it? Then the jab made many more than that sick, disabled, or dead. Almost everything we’ve been told about this over the past three years is a lie.
Thank you for breaking it down for those without a science background, talk about throwing the animals under the bus, is this agenda to rid the world of all living species beside humans? Nope not even going there
I find this post, and the one before on Bayesian analysis, to be the most strikingly objective pieces I've read during the pandemic. Everything else has a spin that can be felt. I sensed that you were truly open to letting the evidence lead you where it will. That's a *real* scientist... Thank you.
I feel I just learned something. Thanks, Alex!
Thank you for explaining this to a laymen audience. Most of the arguments for a lab origin are perfectly understandable for everyone who is willing to do a little effort and keeping an open mind.
It’s ok—no credibility was ruined here.
It was already shot to shit a long time ago.
It seems that the kit that are commercially available does target the mitochondrion for human nucleic acid removal…….
Presumably by primers with minimal overlap? One wonders what other systematic biases are introduced by such a kit. For example, they probably also down weight amplification of primates closely related to humans, and all manners of other animals may likely have variable alignments with their primer set, suggesting the actual relative abundances are meaningless as they are non-identifiably confounded by the artifact of primer binding intensity…
At least for the kit provided by Qiagen https://www.qiagen.com/br/resources/download.aspx?id=45fc5075-8f8d-4bcf-8019-f4ff28abe224&lang=en#page3
(The FastSelect kit), the same company that made the QIAmp viral RNA mini-kit “Qiagen micro-extraction kit”, enrichment of non-human reads are performed through the use of LNA oligomers that binds to and prevent amplification of nucleic acids carrying sequence homology as the oligomers in the kit.
https://www.nature.com/articles/nmeth.f.352
The ILLUMINA Ribo-Zero Gold kit, which is the only available kit from illumina for the same purpose (human NA removal) before the release of the Ribo-Zero plus kit in 04/02/2020, use a probe based pulldown method to remove human rRNA, including mitochondrial rRNA. Both kits selectively removes human reads, and targets the mitochondrion.
https://www.qiagen.com/us/resources/download.aspx?id=c80685c0-4103-49ea-aa72-8989420e3018&lang=en
Neither kits included DNAse I and like the same Qiagen viral RNA kit, don’t distinguish cellular DNA from RNA.
Gao "retired".
Thank you Alex
Another important point is that the PCR assays developed from the work of Christian Drosten in Germany, and widely, if not universally used in the U.S. and elsewhere were not based upon actual genomic sequencing from a human or animal sample, but from a computer model! Cases, Cases! Cases up the wazoo!
They’re cute, though, those raccoon dogs. Most media outlets long ago lost their credibility, as well as social media. My question is, what exactly is this virus? Coronaviruses are ubiquitous in human and animal populations, and can make people sick, though usually not seriously so, and usually seasonally. There were only a few hot spots, with a relatively high fatality rate early on: Wuhan, Lombardy, NYC, the state of Washington. It appears that many of the deaths attributed to this virus throughout the clown show were iatrogenic, caused by the refusal of early treatment with proven re-purposed drugs like the zinc-ionophere hydroxycloroquine or the anti-parssitic ivermectin, the use of ventilators and remdisivir, and the change in the protocol for treating pneumonia (refusing to treat with antibiotics). Clearly, in these hot spots many of those hospitalized were very ill, but this was not the case in most of the world. Viral infectious diseases do not behave like this. Why would a virus behave very differently in a few places, and not everywhere else? Is it possible ,in a lab, to engineer a RNA virus to become both more lethal and easily transmissible? It seems to me a ridiculous idea, since, as you say, RNA is so unstable. Something clearly made those people sick in those hot spots, but what was it? Then the jab made many more than that sick, disabled, or dead. Almost everything we’ve been told about this over the past three years is a lie.